Flow cytometry is one of the most important techniques used in analysis and sorting of cells. It has been widely used in research detecting apoptosis, cell proliferation, immunophenotyping, …etc. It is important to learn about the flow cytometry machine, how to interpret your data and solving common troubleshooting. After finishing this course will be able to run flow cytometry analysis on your own and effectively interpret your data. This course is composed of recorded lectures and one live online laboratory session.
Please note that this is course will be available on 22nd of November and we are currently taking reservations from students. The date and time of the online live session will be confirmed upon agreement with the enrolled students.
In this course
I. Principles of Flow Cytometry:
- Fluidics system.
II. Fluorescence and Fluorophore:
- Fluorescent markers and their purpose.
- Fluorescent proteins.
III. How to analyze your data
- Gates and regions
IV. Controls used in flow cytometry:
V. How to optimize your experiments:
- Sample preparation
- Live/dead cells
- Doublet discrimination.
VI. Multicolour panels.
VII. Online live laboratory session:
- Understanding the basics of flow cytometry technique
- Understanding the importance of each system that the technique relies on (fluidics, optics, and electronics)
- Learning to use a cell sorter (Start up, sorting, and shut down of the equipment)
- Understanding how the equipment performs the measurements
- Learning to interpret the results and understanding the applications of the technique
This course is suitable for: Pharmacists, chemists, biomedical scientists, researchers in addition to undergraduate and postgraduate students.
Senior Lecturer Dr. Giulia De Falco is presenting 5 important lectures about molecular techniques and their application to medicine. …
Real-time PCR, applications and protocol
This course is suitable for beginners who wish to learn about good practices and applications for the polymerase chain reaction (PCR) protocol. Real time PCR (or qPCR) is a commonly used research technique to quantify the transcription of specific genes. This technique is one of the most common techniques used in life science research. At the end of this course you will be able to design your own PCR reaction and analyze your results. This course ends with a multiple-choice exam. You will have to pass the exam to obtain a certificate of completion.
This course includes the following classes:
i. Introduction – Online lecture about the origins of the PCR technique
ii. DNA replication – Online lecture about the step by step process of DNA replication
iii. RNA transcription – Online lecture about the step by step process of RNA transcription
iv. Protein translation – Online lecture about the step by step process of protein translation.
v. RNA extraction protocol – Online lecture explaining the RNA extraction protocols
vi. RT-PCR protocol – Online lecture explaining the cDNA generation protocol
vii. PCR simulation – Online PCR laboratory session.
viii. PCR reaction protocol – Online lecture about the PCR reaction design
Guidelines on writing an abstract and designing a scientific poster
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Basic Cell Culture
Rated 5.00 out of 5Free!
- Essential Lab. Calculations- Part I Free!
Molecular techniques and their application to medicine