Flow cytometry is one of the most important techniques used in analysis and sorting of cells. It has been widely used in research detecting apoptosis, cell proliferation, immunophenotyping, …etc. It is important to learn about the flow cytometry machine, how to interpret your data and solving common troubleshooting. In this course, we provide 2 online recorded sessions over 2 days explaining the fundamentals of flow cytometery. In addition, you will be able to download common protocols used. On the third day, you will learn about the Fluorescence-automated Cell Sorting (FACS) Simulation. You will learn how to sort a pool of Escherichia coli expressing Green Fluorescent Protein (GFP) at different levels after it has been previously engineered. You will set up the equipment, learn how the cytometer sorts the cells, interpret your results, and clean and shut down the equipment.
On Day 1 and 2:
Understand principles of Flow Cytometry.
Fluoresence and Fluorophore.
Controls used in flow cytometry.
How to analyse your data.
How to optimize your experiments.
Creating Multicolour panels.
Common applications of flow cytometry.
Troubleshooting and action required.
Common protocols used.
On Day 3: Using our smart lab practical session you will:
Understanding the basics of flow cytometry technique.
Understanding the importance of each system that the technique relies on (fluidics, optics, and electronics.
Learning to use a cell sorter (Start up, sorting, and shut down of the equipment).
Understanding how the equipment performs the measurements.
Learning to interpret the results and understanding the applications of the technique
You will have to solve simple quiz questions during the practical session in order to ensure that you understand each step.
At the end, you are required to pass an exam in order to obtain a certificate of completion.
Senior Lecturer Dr. Giulia De Falco is presenting 5 important lectures about molecular techniques and their application to medicine. …
Real-time PCR, applications and protocol
This course is suitable for beginners who wish to learn about good practices and applications for the polymerase chain reaction (PCR) protocol. Real time PCR (or qPCR) is a commonly used research technique to quantify the transcription of specific genes. This technique is one of the most common techniques used in life science research. At the end of this course you will be able to design your own PCR reaction and analyze your results. This course ends with a multiple-choice exam. You will have to pass the exam to obtain a certificate of completion.
This course includes the following classes:
i. Introduction – Online lecture about the origins of the PCR technique
ii. DNA replication – Online lecture about the step by step process of DNA replication
iii. RNA transcription – Online lecture about the step by step process of RNA transcription
iv. Protein translation – Online lecture about the step by step process of protein translation.
v. RNA extraction protocol – Online lecture explaining the RNA extraction protocols
vi. RT-PCR protocol – Online lecture explaining the cDNA generation protocol
vii. PCR simulation – Online PCR laboratory session.
viii. PCR reaction protocol – Online lecture about the PCR reaction design
Guidelines on writing an abstract and designing a scientific poster
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Basic Cell Culture
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- Essential Lab. Calculations- Part I Free!
Molecular techniques and their application to medicine