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Basic Cell Culture

5.00 1 Votes
Dr. Nermeen Moustafa

In this lecture, you will learn: – Types of cells. – Identifying cell culture contamination. – How to count the…

Free Course!

Essential Lab. Calculations- Part I

Lab. calculations are important in conducting research. Correct calculations mean accurate experiment, reproducible results, correct interpretations and true reflection of the outcome. Watch watch this video to learn essential lab. calculations (Part I).

Please do not forget to rate this lecture and post a feedback after watching… Thank You!

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Fundamentals of Flow Cytometry- Part I: Principles of Flow Cytometry.

Flow cytometry is one of the most important techniques used in analysis and sorting of cells. It has been widely used in research detecting apoptosis, cell proliferation, immunophenotyping, …etc. It is important to learn about the flow cytometry machine, how to interpret your data and solving common troubleshooting. In this course, we provide 2 online recorded sessions over 2 days explaining the fundamentals of flow cytometery. In addition, you will be able to download common protocols used. On the third day, you will learn about the Fluorescence-automated Cell Sorting (FACS) Simulation. You will learn how to sort a pool of Escherichia coli expressing Green Fluorescent Protein (GFP) at different levels after it has been previously engineered. You will set up the equipment, learn how the cytometer sorts the cells, interpret your results, and clean and shut down the equipment.

On Day 1 and 2:

Understand principles of Flow Cytometry.

Fluoresence and Fluorophore.

Controls used in flow cytometry.

How to analyse your data.

How to optimize your experiments.

Creating Multicolour panels.

Common applications of flow cytometry.

Troubleshooting and action required.

Common protocols used.

On Day 3: Using our smart lab practical session you will:

Understanding the basics of flow cytometry technique.

Understanding the importance of each system that the technique relies on (fluidics, optics, and electronics.

Learning to use a cell sorter (Start up, sorting, and shut down of the equipment).

Understanding how the equipment performs the measurements.

Learning to interpret the results and understanding the applications of the technique

You will have to solve simple quiz questions during the practical session in order to ensure that you understand each step.

At the end, you are required to pass an exam in order to obtain a certificate of completion.